Adenosine Triphosphate (ATP): Beyond Energy—A Systems Bio...

2025-09-27

Explore the multifaceted role of Adenosine Triphosphate (ATP) as a universal energy carrier and dynamic signaling molecule within cellular metabolism research. This article uniquely integrates ATP’s systems-level impact on mitochondrial regulation, proteostasis, and intercellular communication, providing advanced insights for biomedical investigators.

2025-09-26

Discover how the HyperScribe T7 High Yield RNA Synthesis Kit transforms in vitro transcription RNA workflows for advanced studies in mitochondrial metabolism, post-translational regulation, and RNA-based applications. Uncover unique insights and practical strategies for leveraging this kit in frontier metabolic research.

VER 155008: Unraveling Hsp70 Inhibition in Phase Separati...

2025-09-25

Discover how VER 155008, a potent adenosine-derived HSP 70 inhibitor, uniquely illuminates the interplay between chaperone inhibition, liquid-liquid phase separation, and cancer cell survival pathways. This article provides advanced insights and experimental strategies distinct from conventional reviews.

Sildenafil Citrate: Unraveling Proteoform-Selective Modul...

2025-09-24

Explore how Sildenafil Citrate, a selective cGMP-specific phosphodiesterase type 5 inhibitor, enables advanced research into proteoform-selective modulation and native cell signaling environments. This article offers a novel perspective on leveraging PDE5 inhibition for dissecting complex proteoform–drug interactions beyond conventional cardiovascular research.

NHS-Biotin: Advancing Intracellular Multimeric Protein La...

2025-09-23

Explore how NHS-Biotin, an amine-reactive biotinylation reagent, enables precise and efficient intracellular protein labeling for advanced multimeric protein engineering. This article details its unique advantages for biochemical research and highlights methodological considerations for optimal biotinylation of antibodies and proteins.

br Study approval br Author contributions br

2025-03-03

Study approval Author contributions Conflicts of interest Acknowledgements This work was supported by grants of the Youth Backbone Program (to Jianming Ying) of Cancer Hospital, CAMS, Beijing, Beijing Hope Run Special Fund of Cancer Foundation of China (LC2015A06) and the National Natura

Patients with ADA SCID may experience autoimmune AEs after

2025-03-03

Patients with ADA-SCID may experience autoimmune AEs after SCT (and/or during ERT) that are thought to be related to immune dysregulation prior to achieving complete immune reconstitution. The more common autoimmune manifestations include hemolytic anemia, hypothyroidism, and immune thrombocytopenia

Earlier studies revealed an interaction between HSP and ACK

2025-03-03

Earlier studies revealed an interaction between HSP90β and ACK1 [15]. In the proteomic approach, we show that ACK1 associates with HSP90α and HSP90β. Additionally, we confirm the existence of a HSP90-ACK1 complex with co-IP experiments. We further analysed whether an inhibition of HSP90 affected the

The adult muscle type acetylcholine receptor AChR the exempl

2025-03-03

The adult muscle-type Z-Ligustilide receptor (AChR), the exemplar pLGIC, is a heteropentamer comprising two α subunits, and one each of the β, δ, and ɛ subunits (Sine, 2012). These five subunits come together in a counterclockwise α-ɛ-α-δ-β arrangement (Figure 1A). Binding of acetylcholine to the A

STF 31 br Difference between ACh and ER tests There are few

2025-03-03

Difference between ACh and ER tests There are few reports concerning the provoked spasm between ER and ACh in the same patients [51], [52], [53]. Different mediators may have the potential of different coronary responses. In our experience, spasm provoked by intracoronary injection of ER is focal

br Disclaimer br Conflicts of interest br Role of the

2025-03-03

Disclaimer Conflicts of interest Role of the Sponsor Acknowledgements Funding/support: This project was supported by a grant from the Department of Surgery (R5129), Western University and by the Institute for Clinical Evaluative Sciences (ICES) Western site. ICES is funded by an annual g

The inhibition of the exacerbated inflammatory response that

2025-03-03

The inhibition of the exacerbated inflammatory response that follows injury may be an alternative approach to induce a more efficient repair [17,83]. In line with that, 5-LO is one of the most consolidated targets for dampening inflammation, mainly through the therapeutic effects of 5-LO inhibitors

Sildenafil pharmacology The obtained results indicate that

2025-03-03

The obtained results indicate that even when the impairment of the same forms of memory are present, amnesia can develop in different ways. Impairment of memory reconsolidation in the conditioned food aversion model through both the serotonin receptor antagonist and the antagonist of NMDA glutamate

The obtained results indicate that

2025-03-03

The obtained results indicate that even when the impairment of the same forms of memory are present, amnesia can develop in different ways. Impairment of memory reconsolidation in the conditioned food aversion model through both the serotonin receptor antagonist and the antagonist of NMDA glutamate

br Acknowledgment br Introduction The yeast Cdc ATPase and

2025-03-03

Acknowledgment Introduction The yeast Cdc48 ATPase and its metazoan ortholog p97 (or VCP) are critical components of many ubiquitin-dependent cellular pathways that require the segregation of individual proteins from binding partners or membranes (for review, see Buchberger, 2013, Meyer and We